牦牛Smad 4基因mRNA组织表达谱及其miRNAs初步研究

doi:10.11843/j.issn.0366-6964.2017.10.011

Abstract

Abstract:

The aim of this study was to investigate the mRNA expression profile of Smad 4 gene and to explore the possible miRNAs that target to Smad 4 gene in yak. The reverse transcription PCR(RT-PCR) was used to detect the expression of Smad 4 gene mRNA in the 12 yak tissues including hypothalamus,pituitary,liver,heart,spleen,lung,kidney,biceps femoris,lymph nodes,ovary,oviduct and uterus.The possible miRNAs that target to Smad 4 gene were predicted by TargentScan and miRBase softwares, and their tissue expression profiles in yak were also analyzed by RT-PCR. The results showed that Smad 4 mRNA was expressed in the 12 yak tissues, especially widely expressed in the ovary, oviduct and uterus of the reproductive system. It was predicted that there were 20 miRNAs that could target to the Smad 4 gene of yak, and 6 were selected for expression analysis. The results indicated that bta-miR-106a and bta-miR-377 were expressed in the 12 detected tissues, and bta-miR-146a, bta-miR-224 and bta-miR-1434-5p were expressed in other tissues except for the oviduct of yak, whereas bta-miR-212 was expressed only in the ovaries. The results suggest that Smad 4 gene may play important roles in the various tissues of yak, and bta-miR-212 may be a miRNA targeting to Smad 4 gene only in the ovary of yak, which possibly participates in reproductive physiological process in yak, needing further studies to clarify the mechanism.

CLC Number:

S823.8⁺5.2

WANG Yu-heng, XU Ye-fen, NIU Jia-qiang, WANG Hong-hui, SUOLANG Si-zhu, QIANGBA Yang-zong, ZHANG De-cheng, GUO Min, CHENG Ling-hua. Preliminary Studies on Tissue Expression Profiles of Smad 4 mRNA and Its miRNAs in Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1882-1891.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2017.10.011

https://www.xmsyxb.com/EN/Y2017/V48/I10/1882

References

[1] JUENGEL J L, MCNATTY K P. The role of proteins of the transforming growth factor-β superfamily in the intraovarian regulation of follicular development[J]. Hum Reprod Update, 2005, 11(2): 143-160.
[2] ASHRY M, LEE K, MONDAL M, et al. Expression of TGFβ superfamily components and other markers of oocyte quality in oocytes selected by brilliant cresyl blue staining: relevance to early embryonic development[J]. Mol Reprod Dev, 2015, 82(3): 251-264.
[3] CHANG H, BROWN C W, MATZUK M M. Genetic analysis of the mammalian transforming growth factor-β superfamily[J]. Endocr Rev, 2002, 23(6): 787-823.
[4] FINDLAY J K, DRUMMOND A E, DYSON M L, et al. Recruitment and development of the follicle; the roles of the transforming growth factor-β superfamily[J]. Mol Cell Endocrinol, 2002, 191(1): 35-43.
[5] LIN S Y, MORRISON J R, PHILLIPS D J, et al. Regulation of ovarian function by the TGF-beta superfamily and follistatin[J]. Reproduction, 2003, 126(2): 133-148.
[6] HELDIN C H, MIYAZONO K, DIJKE P T. TGF-β signalling from cell membrane to nucleus through SMAD proteins[J]. Nature, 1997, 390(6659): 465-471.
[7] LEE K B, ZHANG K, FOLGER J K, et al. Evidence supporting a functional requirement of SMAD4 for bovine preimplantation embryonic development: a potential link to embryotrophic actions of follistatin[J]. Biol Reprod, 2014, 91(3): 62.
[8] LUUKKO K, YLIKORKALA A, MÄKELÄ T P. Developmentally regulated expression of Smad3, Smad4, Smad6, and Smad7 involved in TGF-beta signaling[J]. Mech Dev, 2001, 101(1-2): 209-212.
[9] WONG C, ROUGIER-CHAPMAN E M, FREDERICK J P, et al. Smad3-Smad4 and AP-1 complexes synergize in transcriptional activation of the c-Jun promoter by transforming growth factor β[J]. Mol Cell Biol, 1999, 19(3): 1821-1830.
[10] ITOH S, TEN DIJKE P. Negative regulation of TGF-β receptor/Smad signal transduction[J]. Curr Opin Cell Biol, 2007, 19(2): 176-184.
[11] BARTEL D P. microRNAs: genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116(2): 281-297.
[12] AMBROS V. The functions of animal microRNAs[J]. Nature, 2004, 431(7006): 350-355.
[13] 徐源, 孙铁成, 张爱玲, 等. 卵巢miRNA研究进展[J]. 畜牧兽医学报, 2014, 45(4): 509-516.
XU Y, SUN T C, ZHANG A L, et al. Progress on the research of ovarian miRNA[J]. Acta Veterinaria et Zootechnica Sinica, 2014, 45(4): 509-516. (in Chinese)
[14] YANG X K, ZHOU Y, PENG S, et al. Differentially expressed plasma microRNAs in premature ovarian failure patients and the potential regulatory function of mir-23a in granulosa cell apoptosis[J]. Reproduction, 2012, 144(2): 235-244.
[15] WANG Y, REN J W, GAO Y, et al. microRNA-224 targets SMAD family member 4 to promote cell proliferation and negatively influence patient survival[J]. PLoS One, 2013, 8(7): e68744.
[16] YAO G D, YIN M M, LIAN J, et al. microRNA-224 is involved in transforming growth factor-β-mediated mouse granulosa cell proliferation and granulosa cell function by targeting Smad4[J]. Mol Endocrinol, 2010, 24(3): 540-551.
[17] YIN M M, LV M R, YAO G D, et al. Transactivation of microRNA-383 by steroidogenic factor-1 promotes estradiol release from mouse ovarian granulosa cells by targeting RBMS1[J]. Mol Endocrinol, 2012, 26(7): 1129-1143.
[18] YAN G J, ZHANG L X, FANG T, et al. microRNA-145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB[J]. FEBS Lett, 2012, 586(19): 3263-3270.
[19] LIN F, LI R, PAN Z X, et al. miR-26b promotes granulosa cell apoptosis by targeting ATM during follicular atresia in porcine ovary[J]. PLoS One, 2012, 7(6): e38640.
[20] 张小辉. 牛9个繁殖性状相关基因cDNA克隆、SNPs及组织表达研究[D]. 杨凌: 西北农林科技大学, 2007.
ZHANG X H. cDNA clone, SNPs and tissues expression analysis of 9 genes on cattle reproduction trait[D]. Yangling: Northwest A&F University, 2007. (in Chinese)
[21] SHI R, CHIANG V L. Facile means for quantifying microRNA expression by real-time PCR[J]. BioTechniques, 2005, 39(4): 519-525.
[22] LEWIS B P, SHIH I H, JONES-RHOADES M W, et al. Prediction of mammalian microRNA targets[J]. Cell, 2003, 115(7): 787-798.
[23] LEWIS B P, BURGE C B, BARTEL D P. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets[J]. Cell, 2005, 120(1): 15-20.
[24] 张小辉, 张路培, 许尚忠, 等. 牛Smad4基因cDNA克隆及生物信息学分析[J]. 畜牧兽医学报, 2007, 38(4): 321-325.
ZHANG X H, ZHANG L P, XU S Z, et al. Cloning and bioinformaton analysis of cDNA encoding cattle Smad4 gene[J]. Acta Veterinaria et Zootechnica Sinica, 2007, 38(4): 321-325. (in Chinese)
[25] SIRARD C, DE LA POMPA J L, ELIA A, et al. The tumor suppressor gene Smad4/Dpc4 is required for gastrulation and later for anterior development of the mouse embryo[J]. Genes Dev, 1998, 12(1): 107-119.
[26] HUANG Z, YUAN X, WANG M, et al. Molecular cloning of the SMAD4 gene and its mRNA expression analysis in ovarian follicles of the Yangzhou goose (Anser cygnoides)[J]. Br Poult Sci, 2016, 57(4): 515-521.
[27] XU Y F, LI E L, HAN Y D, et al. Differential expression of mRNAs encoding BMP/Smad pathway molecules in antral follicles of high-and low-fecundity Hu sheep[J]. Anim Reprod Sci, 2010, 120(1-4): 47-55.
[28] MIAO Z L, WANG Z N, CHENG L Q, et al. Expression of Smad4 during rat ovarian development[J]. J First Mil Med Univ, 2005, 25(2): 127-131.
[29] MOSKOWITZ I P, WANG J, PETERSON M A, et al. Transcription factor genes Smad4 and Gata4 cooperatively regulate cardiac valve development[J]. Proc Natl Acad Sci U S A, 2011, 108(10): 4006-4011.
[30] MAO X, DEBENEDITTIS P, SUN Y, et al. Vascular smooth muscle cell Smad4 gene is important for mouse vascular development[J]. Arterioscler Thromb Vasc Biol, 2012, 32(9): 2171-2177.
[31] YANG L L, MAO C M, TENG Y, et al. Targeted disruption of Smad4 in mouse epidermis results in failure of hair follicle cycling and formation of skin tumors[J]. Cancer Res, 2005, 65(19): 8671-8678.
[32] PANGAS S A, LI X H, ROBERTSON E J, et al. Premature luteinization and cumulus cell defects in ovarian-specific Smad4 knockout mice[J]. Mol Endocrinol, 2006, 20(6): 1406-1422.
[33] YU C, ZHANG Y L, FAN H Y. Selective Smad4 knockout in ovarian preovulatory follicles results in multiple defects in ovulation[J]. Mol Endocrinol, 2013, 27(6): 966-978.
[34] LIANG M, YAO G D, YIN M M, et al. Transcriptional cooperation between p53 and NF-κB p65 regulates microRNA-224 transcription in mouse ovarian granulosa cells[J]. Mol Cell Endocrinol, 2013, 370(1-2): 119-129.
[35] YAO G D, LIANG M, LIANG N, et al. microRNA-224 is involved in the regulation of mouse cumulus expansion by targeting Ptx3[J]. Mol Cell Endocrinol, 2014, 382(1): 244-253.
[36] LI X F, WANG H D, SHENG Y, et al. microRNA-224 delays oocyte maturation through targeting Ptx3 in cumulus cells[J]. Mech Dev, 2017, 143: 20-25.
[37] HUSZAR J M, PAYNE C J. microRNA 146 (Mir146) modulates spermatogonial differentiation by retinoic acid in mice[J]. Biol Reprod, 2013, 88(1): 15.
[38] FENG B, DONG T T, WANG L L, et al. Colorectal cancer migration and invasion initiated by microRNA-106a[J]. PLoS One, 2012, 7(8): e43452.
[39] ZHU M, ZHANG N, HE S X, et al. microRNA-106a targets TIMP2 to regulate invasion and metastasis of gastric cancer[J]. FEBS Lett, 2014, 588(4): 600-607.
[40] ZHU M, ZHANG N, HE S X, et al. microRNA-106a functions as an oncogene in human gastric cancer and contributes to proliferation and metastasis in vitro and in vivo[J]. Clin Exp Metastasis, 2016, 33(5): 509-519.
[41] MENG F, ZHANG L, SHAO Y, et al. microRNA-377 inhibits non-small-cell lung cancer through targeting AEG-1[J]. Int J Clin Exp Pathol, 2015, 8(11): 13853-13863.

Preliminary Studies on Tissue Expression Profiles of Smad 4 mRNA and Its miRNAs in Yak

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	A GUO Yueda, XIONG Xianrong, WANG Yan, YANG Xianying, HAN Jie, WANG Bin, LI Jian. Identification and Analysis of Long Non-coding RNA Associated with Cattle-yak Male Infertility [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 551-561.
[2]	FANG Lili, YANG Jinfang, JING Haixia, YU Hongxian, ZHANG Qinwen, LI Li, WEI Qing, WANG Xinyue, LI Feng, CHEN Yonghao, YANG Yuying, LI Guangzhen. Distribution and Expression of Estrogen Receptors in Mammary Gland of Yak during Mid-lactating Period and Non-lactating Period [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 211-217.
[3]	HAN Jie, XIONG Xian-rong, WANG Yan, YANG Xian-ying, A GUO Yue-da, HUANG Xiang-yue, LI Jian. Cloning of Yak KDM1A Gene and Its Expression Profile in Testis during Different Developmental Stages [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2777-2785.
[4]	TANG Peng, LING Xiao-xiao, JIA Cong-jun, LIANG Chun-nian, WU Xiao-yun, WANG Hong-bo, CHU Min, YAN Ping. Study on the Differences of HSP70 Expression in Fresh Sperm and Frozen-thawed Sperm in Yak [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2514-2520.
[5]	TANG Peng, LING Xiao-xiao, GAO Ze-cheng, JIA Cong-jun, LIANG Chun-nian, WU Xiao-yun, CHU Min, YAN Ping. Skin Histological Observation of Yak with Different Coat Colors and MC1R Functional Verification [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1377-1386.
[6]	MA Jun-xing, HE Jun-feng, CUI Yan, ZHANG Qian, LIU Peng-gang. The Distribution of LRP6 and VEGFR2 in Yak Lungs [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1550-1557.
[7]	PU Si-ying, ZHENG Jie, YANG Yuan-xiao, WANG Qin, YANG Rao-fen, ZI Xiang-dong. Comparative Transcriptome Analysis between Fresh and Vitrified-thawed Blastocysts of the Yak (Bos grunniens) [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 709-717.
[8]	CAI Wen-yi, XIONG Xian-rong, CHEN Tong, YANG Xian-ying, HAN Jie, AGUO Yue-da, LI Jian. Molecular Cloning of KDM2B Gene and Its Expression Pattern in Tissues and the Process of Oocyte Meiosis in Yak [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 534-541.
[9]	HAN Jie, XIONG Xian-rong, CAI Wen-yi, YANG Xian-ying, A GUO Yue-da, ZHANG Yan-hong, LI Jian. Cloning of KDM4A Gene and Its Expression in Different Tissues, Oocyte and Granulosa Cell of Yak [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 291-299.
[10]	WANG Ya-ying, PAN Yang-yang, YE Xiao-lin, LI Gu-yue, HE Hong-hong, WANG Meng, FAN Jiang-feng, CUI Yan, YU Si-jiu. Cloning of Yak Cyclin D1 Gene and the Effects of IGF-I on Its Expression in Sertoli Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 310-317.
[11]	ZI Xiang-dong, LIU Shuang, XIA Wei, XIONG Xian-rong, HUANG Lin, ZHANG Zheng-fan, LI Zhi-xiong, LI Jian, ZHONG Jin-cheng, WANG Li, ZHU Jiang-jiang. Transcriptome Analysis during Development of Hybrid in vitro-derived Yak Embryos [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 92-101.
[12]	ZHENG Jie, PU Si-ying, YANG Yuan-xiao, WANG Qin, YANG Rao-fen, ZI Xiang-dong. Exploring Mechanism for Vitrification Damage of the Cross-bred Blastocysts of the Yak via High-throughput Sequencing [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1871-1881.
[13]	PAN Yang-yang, WANG Meng, YU Si-jiu, CUI Yan, HE Jun-feng. The Effects of EGF on Yak Frozen Sperm Motility, Apoptosis and the Development Competence of Embryos after IVF [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 854-862.
[14]	ZHAO Tian，LI Gu-yue，PAN Yang-yang，LI Yue-qin，CHEN Ping，WEN Ze-xing，CUI Yan， YU Si-jiu. The Expression of Octamer-binding Transcription Factor 4 Gene (Oct 4) in Yak (Bos grunneins) Oocytes and Preimplantation Embryos Produced by in vitro Fertilization [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(3): 577-584.
[15]	LI Li，LIU Meng-qi，HU Pei-ying，ZHANG Qin-wen，YU Hong-xian，JING Hai-xia. The Succinate Dehydrogenase Genetic Analysis of Placenta and Fetus Tissue Mitochondria in Datong Yak [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 284-289.